Download e-book for kindle: Analysis of Neuropeptides by Liquid Chromatography and Mass by Dominic M. Desiderio

By Dominic M. Desiderio

ISBN-10: 0444424180

ISBN-13: 9780444424181

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Extra resources for Analysis of Neuropeptides by Liquid Chromatography and Mass Spectrometry

Example text

The amygdaloid nuclei constitute a prominent group of structures within the limbic system and contain the highest densities of opiate receptors and enkephalins found in the brain. Other than the intestine, enkephalins have not been detected outside the CNS. Enkephalins may play a role in the intestine by their ability to combat diarrhea and cause constipation. Opiates are also known to constrict the eye pupils and indeed, the pinpoint pupils of heroin addicts is a simple law enforcement method to assess a potential drug user.

T h e a u t h o r s s t a t e a n important consideration t h a t t h e m e r e detection of a p e p t i d e i n a t i s s u e e x t r a c t should not b e t a k e n a s d i r e c t evidence t h a t t h a t p e p t i d e r e p r e s e n t s a n actual physiological p r o d u c t . T h i s conclusion i s a n important conceptual consideration because a n intermediate, incomplete inactivation of a p i t u i t a r y enzyme d u r i n g extraction o r else post-mortem autolysis may possibly p r o d u c e some of t h e detected peptides in a n artifactual manner.

The opioid activity in CSF samples obtained from patients undergoing diagnosis for intracranial hydrodynamic dysfunction were fractionated (Sephadex C10) and electrophorctically scparated. The characteristics of endorphins identified by RIA and R R A were compared (89b). While RIA did not detect any of the opioid peptides, R R A detected a complex pattern of opioid receptor-active peptides, some of which seem to be metabolically related to each other. 5. Peptides tentatively identified and measured in human CSF (89a).

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Analysis of Neuropeptides by Liquid Chromatography and Mass Spectrometry by Dominic M. Desiderio


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